Clinical significance of the expression of pulmonary surfactant associated protein in bronchoalveolar lavage fluid of children with refractory Mycoplasma pneumoniae pneumonia / 新乡医学院学报

Objective To investigate the clinical significance of the expression of pulmonary surfactant associated pro-tein(SP)in bronchoalveolar lavage fluid(BALF)of children with refractory Mycoplasma pneumoniae pneumonia(RMPP). Methods Thirty children with RMPP were selected from January 2015 to December 2016 in the People's Hospital of Hebi City. The lung function of the children was detected in acute and recovery stage,and bronchoalveolar lavage was performed with fiexible bronchofiberscope. The BALF was collected,and the levels of SP-A,SP-B,SP-C and SP-D in BALF were detected by enzyme linked immunosorbent assay. Results The forced expiratory volume in one second (FEV 1 ),forced vital capacity (FVC)and FEV1 / FVC in RMPP children at acute stage were (1. 34 ± 0. 23)L,(1. 75 ± 0. 28)L and (68. 25 ± 6. 21)％respectively;and they were (1. 71 ± 0. 35)L,(1. 98 ± 0. 36)L and (88. 57 ± 8. 16)％ respectively in the children at recov-ery stage. The FEV1 ,FVC and FEV 1 / FVC in RMPP children at recovery stage were significantly higher than those in the chil-dren at acute stage (t = 4. 839,3. 070,14. 859;P < 0. 05). The levels of SP-A,SP-B,SP-C and SP-D in the RMPP children at acute stage were (50. 19 ± 10. 06),(42. 95 ± 12. 42),(36. 81 ± 8. 14)and (21. 57 ± 5. 46)μg·L - 1 respectively;and they were (135. 20 ± 18. 13),(108. 42 ± 20. 33),(142. 63 ± 21. 87)and (72. 69 ± 8. 54)μg·L - 1 respectively in the children at recovery stage. The levels of SP-A,SP-B,SP-C and SP-D in BALF of RMPP children at recovery stage were significantly higher than those in the children at acute stage (t = 22. 457,15. 052,24. 837,27. 623;P < 0. 05). Conclusion The detection of SP-A,SP-B,SP-C and SP-D levels in BALF plays a guiding role in the diagnosis,disease assessment,treatment and prognosis judgment of RMPP.

Relationship between surfactant associated protein-A in bronchoalveolar lavage fluid and lung function in children with refractory mycoplasma pneumoniae pneumonia / 新乡医学院学报

Objective To investigate the relationship between surfactant associated protein-A(SP-A) in bronchoalveolar lavage fluid(BLAF) and lung function in children with refractory mycoplasma pneumoniae pneumonia(RMPP).Methods Thirty children with RMPP were selected from January 2015 to December 2016 in the People's Hospital of Hebi City.The partial pressure of oxygen in artery (PaO2),partial pressure of carbon dioxide in artery (PaCO2),forced expiratory volume in one second(FEV1) and forced vital capacity (FVC) of the children were detected at acute and convalescent periods.The BALF was collected by bronchoalveolar lavage,and the level of SP-A in BALF was detected by enzyme linked immunosorbent assay.Results The level of SP-A in BALF of children with RMPP at ac ute and convalescent period was (3.63 ± 0.09) and (5.86 ± 0.17)mg · L-1 respectively,the level of SP-A in BALF of children with RMPP at acute phase was significantly lower than that at convalescent period(t =-63.499,P ＜ 0.05).The PaO2 and PaCO2 in children with RMPP at acute phase were (49.25 ±7.32) and (47.16 ±6.48)mmHg respectively,and they were (76.54 ±6.48) and (36.20 ± 5.61)mmHg respectively at convalescent period;the PaO2 in children with RMPP at acute phase was significantly lower than that at convalescent period (t =-15.289,P ＜ 0.05),and the PaO2 in children with RMPP at acute phase was significantly higher than that at convalescent period(t =7.004,P ＜ 0.05).The FEV1,FVC and FEV1/FVC in RMPP children at acute phase were (1.36 ±0.67),(1.68 ± 0.31) L and 69.85 ± 8.34 respectively;and they were (1.89 ± 0.58),(1.99 ± 0.53) L and 87.32 ± 9.52 respectively at convalescent period;the FEV1,FVC and FEV1/FVC in RMPP children at acute phase were significantly lower than those at convalescent period(t =-3.276,-2.765,-7.560;P ＜ 0.05).The level of SP-A in BALF of children with RMPP was positively correlated with PaO2 (r =0.921 6,P ＜ 0.05),but there was no significant correlation between SP-A level and PaCO2 (r =1.211 4,P ＜ 0.05).The level of SP-A in BALF was positively correlated with FEV1,FVC and FEV1/FVC (r =0.831,0.905,0.803;P ＜ 0.05).Conclusion The level of SP-A in BALF was positively correlated with lung function of children with RMPP.The detection of SP-A level in BALF is helpful to assess the lung function and pathogenetic condition of children with RMPP.

Effects of silencing chloride intracellular channel 1 gene expression on the proliferation and invasion of mouse hepatocellular carcinoma cell lines / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To study the effects of silencing CLIC1 gene expression on the proliferation and invasion of Hca-F cells.</p><p><b>METHODS</b>The mouse CLIC1 cDNA sequence was retrieved from NCBI. Three shRNA sequences were designed and cloned into pGPU6/GFP/Neo plasmids. The plasmids were transfected into Hca-F cells with Lipofectamine 2000. Cell Counting-8 (CCK-8) kit and transwell chamber were used to study the effects of CLIC1 on the proliferation and invasion of Hca-F cells.</p><p><b>RESULTS</b>The pGPU6/GFP/Neo-shRNA-3 plasmid effectively repressed the expression of CLIC1 mRNA. Inhibition of CLIC1 gene expression led to decreased cell proliferation and reduced invasion.</p><p><b>CONCLUSION</b>CLIC1 is essential for the proliferation and invasion of Hca-F cells.</p>

Experimental study on monitoring gene expression by noninvasive method in rabbit VX2 liver tumor model / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To investigate the feasibility of monitoring therapeutic effect of adenovirus vector containing IL12-IRES-CKb gene on a rabbit VX2 liver tumor model by using phosphorous-31 magnetic resonance spectroscopy (31P MRS).</p><p><b>METHODS</b>A total of 18 healthy New Zealand White rabbits were used to generate animal models by implanting VX2 tumor chips into livers through laparotomy. Tumor-bearing animals were randomly divided into three groups and were injected with AdCMVIL12-IRES-CKb, AdCMV-Empty and saline respectively via ear veins. 31P MRS scan was performed after animals were fed with creatine solution for five days. Animals were euthanized thereafter and tumors were removed for pathological examination, immunohistochemistry (IHC) staining and protein analysis (Western blot).</p><p><b>RESULTS</b>The intrahepatic and seral expressions of creatine kinase (CKb) and IL-12 were detected only in AdCMVIL12-IRES-CKb group. Tumor diameters pre- and post- treatment in three groups were 1.63+/-0.04 vs 1.62+/-0.03 in AdCMVIL12-IRES-CKb group (P = 0.229), 1.59+/-0.05 vs 1.84+/-0.11 in AdCMV-Empty group (P = 0.003) and 1.60+/-0.02 vs 2.07+/-0.12 in saline group (P = 0.001), respectively. Pcr Changes between pre- and post- treatment among the three groups were compared (F = 6.235, P value is less than 0.05). PCr increased significantly in AdCMVIL12-IRES-CKb group as compared to AdCMV-Empty (P = 0.004) and saline group (P = 0.049), whereas no change found between AdCMV-Empty and saline group (P = 0.153).</p><p><b>CONCLUSION</b>31P MRS, an effective and non-invasive functional imaging method, can be used to monitor the therapeutic effect of adenovirus vector containing IL12-IRES-CKb gene on rabbit VX2 liver tumor model through detecting metabolic product of imaging reporter gene CKb (pCr).</p>